Evaluate adding green seaweed to different rations by In vitro gas production technique

Abstract

In vitro gas production technique used to evolution supplemented green seaweed (Ulva lactuca) with different levels on two rations containing rice straw and concentrate and effects on some rumen parameters, digestibility, degradability (DM, OM, NDF, ADF, hemicellulose and cellulose) and gas production values. Three rations were used to In vitro techniques and the rations were: R1(standard ration):40% clover hay + 60%concentrate, R2: 40% rice straw + 60%concentrate, R3: 50% rice straw + 50% concentrate. Supplemented green seaweed (Ulva lactuca) with different levels were (0,0.8,1.6,2.4 and 3.2% from DM ration). The results indicated that the rate of fermentation gas production was high in all rations adding green seaweed. Ruminal pH and rate gas production per hour (Rate GP / h) was not affected during fermentation processes, Short chain fatty acid ( SCFA ) and metabolic energy (ME ) cleared no significant differences among all different adding and control groups in the experiment. Ammonia concentration recorded the lowest value of ammonia in R2(1.6%) and R3 (0.8%). Microbial protein (MP) and efficiency microbial protein ( EMP) recorded high values in all different addition of green seaweed compared control (0%). The highest significant value of DMD (p<0.05) was found in R2 adding seaweed 0.8 % and 3.2% (63.21 and 63.54 %). OMD in R2 recorded the highest values in all supplementation especially level of 3.2% compared 0% and R1, but in R3 the level of 0.8%adding seaweed was the highest value only. It was noticed, that the values of hemicellulose digestibility increased with increase in the level of green seaweed compared 0 %. Cellulose digestibility (Cellul. D) values were recorded the highest value in all adding green seaweed especially level 3.2 in R2 and level 1.6 in R3 (76.58 and 48.92 %, respectively) compared with R1 (28.84%). Degradability of DM, OM, NDF, ADF, Hemicell and Cellu. were increasing with adding green seaweed with different levels supplementation in R2 especially added 3.2% was the highest value compared with 0% added. In R3 the highest value was adding 0.8% green seaweed only but any adding were low values compared 0% added. It concluded that supplementation seaweed (Ulvalactuca) with different levels due to improving digestibility, kinetics of gas production, growth of microbial protein biomass, efficiency of microbial protein and degradability especially R2 containing 40% rice straw but R3 containing 50% rice straw in one level adding 0.8% DM and equaled values standard ration (R1) containing clover hay.