Attenuation of Aspirin-Induced Gastric Ulcer in Rats by Linseed, Pumpkin and Lupine seed oils

Abstract

This study aimed to investigate, whether the oils of linseed, pumpkin and lupine seeds can heal the gastric ulcer produced by aspirin (ASP) in rats compared to ranitidine drug. F¬¬orty eight male Sprague Dawely rats (150-200 g) were divided into 6 groups (8 rats /group). Group 1 was normal control; the other groups were treated with ASP (400 mg/kg body weight, p.o). In addition to ASP, group 2 (positive +ve, control) received saline, group 3 was treated with ranitidine (50 mg/kg body weight, p.o), and groups from 4 to 6 were treated daily with one of the three tested oils (1.5 ml/kg body weight, p.o) for 7 days. The volume of gastric juice, total acidity of gastric secretion and ulcer index were significantly reduced while the pH of the gastric fluid was significantly increased in the oils treated groups, compared with the +ve control. These results were associated with significant increase in serum total antioxidant capacity (TAC) and a reduction in total oxidant capacity (TOC). Also, The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β), were suppressed after randitine or oils treatment. The gastric prostaglandin E2 (PGE2) and cytochrome P450 reductase (CYP450 red) levels were also increased in ranitidine or each of pumpkin and linseed oils treated groups, whereas gastric cyclooxygenase 2 (COX-2) and total nitric oxide (NO) were significantly reduced in ranitidine and linseed oil treated groups. The best improvement was found in the group treated with ranitidine followed by linseed oil group. These results suggest that the three tested oils attenuated the aspirin-induced gastric lesions via antisecretory, antiulcer, antioxidant and anti-inflammatory mechanisms.