Ameliorative Effect of Flavonoid Antioxidant on the Histology and Ultrastructure of Albino Rats` Liver Treated with Radiation and /or Acrylonitrile

Abstract

The present study was established to investigate the effect of acrylonitrile toxicity or/and -irradiation on albino rats liver tissue and the role of hesperidin in recovery and improvement. eighty male albino rats (100-110gm) were divided into eight groups. Control group received orally a mixture of dimethyl sulphoxide and water (1: 9, v/v); Acrylonitrile treated group was given daily dose of (50mg/kg body weight)/day for 4 weeks; Acrylonitrile or/and group exposed to whole body -irradiation up to dose of 6Gy accumulative dose; Hesperidin treated group received daily dose of (100 mg/kg body weight) for 4 weeks; hesperidin and acrylonitrile daily dose of (50mg/kg body weight)/day and/or -irradiation up to dose of 6Gy accumulative dose (1.5Gy/ week). Light and transmission electron microscopic were used to examine liver tissue. The histological investigation showed deterioration in the liver's tissues pattern in the acrylonitrile treated group or/and -irradiated one. The results obtained, showed an improvement in liver tissue in the groups treated with hesperidin. Such results indicated that co-administration of hesperidin was found to be more effective in restoring, histological and electron microscopic alterations.