Bone marrow-derived mesenchymal stem cells ameliorate sodium nitrite-induced hypoxic brain injury in a rat model
Amany Abdelhamied Mahmoud Osman; Omar A. Ahmed-Farid2; Elham H A Ali;
Abstract
Sodium nitrite (NaNO2) is an inorganic salt used broadly in chemical industry. NaNO2 is highly reactive
with hemoglobin causing hypoxia. Mesenchymal stem cells (MSCs) are capable of differentiating into a
variety of tissue specific cells and MSC therapy is a potential method for improving brain functions. This
work aims to investigate the possible therapeutic role of bone marrow-derived MSCs against NaNO2
induced hypoxic brain injury. Rats were divided into control group (treated for 3 or 6 weeks), hypoxic
(HP) group (subcutaneous injection of 35 mg/kg NaNO2 for 3 weeks to induce hypoxic brain injury), HP
recovery groups N-2wR and N-3wR (treated with the same dose of NaNO2 for 2 and 3 weeks respectively,
followed by 4-week or 3-week self-recovery respectively), and MSCs treated groups N-2wSC and N-3wSC
(treated with the same dose of NaNO2 for 2 and 3 weeks respectively, followed by one injection of 2 ×
106 MSCs via the tail vein in combination with 4 week self-recovery or intravenous injection of NaNO2
for 1 week in combination with 3 week self-recovery). The levels of neurotransmitters (norepinephrine,
dopamine, serotonin), energy substances (adenosine monophosphate, adenosine diphosphate, adenosine
triphosphate), and oxidative stress markers (malondialdehyde, nitric oxide, 8-hydroxy-2′-deoxyguanosine,
glutathione reduced form, and oxidized glutathione) in the frontal cortex and midbrain were measured
using high performance liquid chromatography. At the same time, hematoxylin-eosin staining was
performed to observe the pathological change of the injured brain tissue. Compared with HP group, pathological
change of brain tissue was milder, the levels of malondialdehyde, nitric oxide, oxidized glutathione,
8-hydroxy-2′-deoxyguanosine, norepinephrine, serotonin, glutathione reduced form, and adenosine triphosphate
in the frontal cortex and midbrain were significantly decreased, and glutathione reduced form/
oxidized glutathione and adenosine monophosphate/adenosine triphosphate ratio were significantly
increased in the MSCs treated groups. These findings suggest that bone marrow-derived MSCs exhibit neuroprotective
effects against NaNO2-induced hypoxic brain injury through exerting anti-oxidative effects
and providing energy to the brain.
with hemoglobin causing hypoxia. Mesenchymal stem cells (MSCs) are capable of differentiating into a
variety of tissue specific cells and MSC therapy is a potential method for improving brain functions. This
work aims to investigate the possible therapeutic role of bone marrow-derived MSCs against NaNO2
induced hypoxic brain injury. Rats were divided into control group (treated for 3 or 6 weeks), hypoxic
(HP) group (subcutaneous injection of 35 mg/kg NaNO2 for 3 weeks to induce hypoxic brain injury), HP
recovery groups N-2wR and N-3wR (treated with the same dose of NaNO2 for 2 and 3 weeks respectively,
followed by 4-week or 3-week self-recovery respectively), and MSCs treated groups N-2wSC and N-3wSC
(treated with the same dose of NaNO2 for 2 and 3 weeks respectively, followed by one injection of 2 ×
106 MSCs via the tail vein in combination with 4 week self-recovery or intravenous injection of NaNO2
for 1 week in combination with 3 week self-recovery). The levels of neurotransmitters (norepinephrine,
dopamine, serotonin), energy substances (adenosine monophosphate, adenosine diphosphate, adenosine
triphosphate), and oxidative stress markers (malondialdehyde, nitric oxide, 8-hydroxy-2′-deoxyguanosine,
glutathione reduced form, and oxidized glutathione) in the frontal cortex and midbrain were measured
using high performance liquid chromatography. At the same time, hematoxylin-eosin staining was
performed to observe the pathological change of the injured brain tissue. Compared with HP group, pathological
change of brain tissue was milder, the levels of malondialdehyde, nitric oxide, oxidized glutathione,
8-hydroxy-2′-deoxyguanosine, norepinephrine, serotonin, glutathione reduced form, and adenosine triphosphate
in the frontal cortex and midbrain were significantly decreased, and glutathione reduced form/
oxidized glutathione and adenosine monophosphate/adenosine triphosphate ratio were significantly
increased in the MSCs treated groups. These findings suggest that bone marrow-derived MSCs exhibit neuroprotective
effects against NaNO2-induced hypoxic brain injury through exerting anti-oxidative effects
and providing energy to the brain.
Other data
Title | Bone marrow-derived mesenchymal stem cells ameliorate sodium nitrite-induced hypoxic brain injury in a rat model | Authors | Amany Abdelhamied Mahmoud Osman ; Omar A. Ahmed-Farid2 ; Elham H A Ali | Keywords | nerve regeneration; hypoxia; bone marrow-derived mesenchymal stem cells; sodium nitrite; monoamine neurotransmitter; cell energy; neural regeneration | Issue Date | Dec-2017 | Journal | NEURAL REGENERATION RESEARCH | Description | This suggests that MSCs alleviated brain injury induced by hypoxia and stimulated the antioxidant defence to promote neuron function and nervous system performance. Histological results revealed that stem cells prevented against neurotoxicity due to hypoxic NaNO2 effects on rat brain, and improved the pathological features of neurodegenerative disorders.The stem cell therapy holds promise for the treatment of a wide range of neurological disorders. As the number and type of regenerative cells have not been identified in this study, future studies are required. |
ISSN | 1673-5374 | DOI | 10.4103/1673-5374.221155 |
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