Midazolam Impact on the Histological and Ultrastructural Characteristics of Mice Adrenal Cortex

Abstract

Midazolam is a member of the benzodiazepine group of tranquilizers. The present study alterations induced by administration of midazolam on the adrenal cortex of male albino mice. Twenty adult male CD study. control. The second group received daily double the therapeutic dose (0.26 mg/kg b.wt.) of midazolam for 28 days. The animals were sacrificed and adrenal samples were obtained and processed for histological and ultrastructural examina midazolam three cortical zones; zona glomerulosa, fasiculata and reticularis, beside the nuclei of some of these cells showing signs of pyknosis, karyolysis. Ultrastructurally, revealed conspicuous alterations, represented by deformed mitochondria with abnormal typ endoplasmic reticulum was endoplasmic reticulum was dilated. The vacuolated cytoplasm contained numerous lysosomes, in addition to numerous lipid droplets. The nuclei showed clear signs of pyknosis and possessed irregular nuclear envelope In conclusion, it seems that the destructive impacts of midazolam on the adrenocortical cells reflected on their functions leading to much deficiency in their performance. So, it should be taken in the consideration and great concern that much drug must medical fields to protect the human health from its hazardous impact.