Protective Effect of Morin against Flutamide-Induced Hepatotoxicity in Mal Wister Rats

Abstract

Aim of the work: the aim of this work was to investigate the protective role of morin against the hepatotoxic effect of flutamide (FLU) that is widely used drug for treatment of metastatic prostate adenocarcinoma. Administration of flutamide to male rats in a dose of 100 mg/ kg b.w. daily for 4 weeks resulted in serious hepatic injury. Materials and Methods: Male Wistar rats were equally divided into six experimental groups (n = 10): Group I (control group) received appropriate vehicle (carboxy methyl cellulose, CMC) for 8 weeks, Group II (CM group ) received CMC for 4 weeks then morin for another 4 weeks, Group III ( M group) were treated with morin for 8 weeks, Group IV (CF group) received CMC for 4 weeks then FLU for another 4 weeks, Group V (CMF group) received CMC for 4 weeks then morin for another 4 weeks then received morin associated with FLU for additional 4 weeks and Group VI (MMF group) was pretreated with morin for 4 weeks then treated with morin simultaneously with FLU for additional 4 weeks. Results: In FLU treated rats, highly significant increases in each of serum ALT, AST and direct and total bilirubin as well as hepatic MDA were observed relative to the control group. Moreover, highly significant decrease in hepatic SOD, GSH and GST activities was observed. On the other hand, administration of morin with FLU resulted in mild and marked reduction in the elevated plasma ALT, AST, direct and total bilirubin and hepatic MDA levels induced by FLU intoxication, respectively (regarding co-treatment and pretreatment with FLU). Conclusion: These data showed protective effect of morin against FLU-induced hepatic damage, especially when administered prior to and concomitantly with FLU.