COMPARATIVE STUDY BETWEEN OXYGEN THERAPY AND RUTIN AS AN ANTIOXIDANT IN HYPERLIPIDEMIC RATS

Abstract

Obesity is a metabolic disorder and fundamental cause of other fatal diseases including atherosclerosis and cancer. One of the main factor that contributes to the development of obesity is high-fat (HF) consumption. In the present study, the changes in selected biochemical blood variables which are thought to represent risk factors coincident with obesity were compared between a group of normal control male albino rats and other groups suffering from obesity induced by feeding rats on fatty diet (fat 50 % diet). Also, histological studied on the liver were involved. In addition, the effects of two antioxidants (rutin and ozonized water) on the same variables were tested and followed in order to examine to what extent, they are valid to control the levels of these variables without any deleterious effects after treatment. Rutin or ozonized water were daily received orally for one and two months in two groups of obese rats in the following doses 50 mg rutin /kg b.wt/day and 0.5 ml ozonized water /kg b.wt/day, respectively. Fasting blood samples were drawn after one and two months at the terminal of the treatments. The obtained results revealed that induced obesity caused significant (p<0.05) increase of serum leptin, resistin, cholesterol, triglycerides, high density lipoprotein (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch), very low density lipoprotein-cholesterol (VLDL- Ch) and phospholipids as compared with their relevant level in normal control rats group. On the other hand, induced obesity in rats caused significant (p<0.05) decrease in the levels of serum free triiodothyronine (FT3). No remarkable changes occurred in the concentrations of serum free thyroxin (FT4). Histological examination of liver tissue after HFD feeding showed histological changes illustrated by necrotic cells; Kupffer cells proliferation, fatty degeneration and loss of hepatic architecture. Moreover, all these changes were supported by increase of DNA damage in hepatocytes. A marked correction following treatments of obese rats with rutin or ozonized water for one and two months occurred in all previous parameters depending on the time of treatment. The best amelioration occurred in the obese rats group which received the ozonized water at the last interval (60 days). The underlining mechanisms were discussed according to available references