Isolation and sequence analysis of a putative MerR-type-transcriptional regulator and a multidrug efflux protein of Bacillus circulans ATCC 21588: as potential targets of therapeutics
Aboshanab K.; Elshafey Mostafa;
Abstract
Mercury-type transcriptional regulators (MerR-transcriptional regulator) and major facilitator
superfamily (MFS) transporters usually form an important sensor-response transport system in many
microorganisms. This system has been shown to be involved in the regulation and transport (efflux) of a
wide and diverse array of secondary metabolites including antimicrobial agents, dyes, chemicals,
metals and evem harmful oxygen radicals. Inhibition or inactivation of this transport system is
considered a promising approach for controlling microbial resistance, and thus may become a
promising target of therapeutics particularly for the clinically relevant pathogens. However, the genetic
and proteomics of this system have not been fully studied. In this work, a DNA segment (1.926 kb) from
Bacillus circulans ATCC 21588 harboring the two genes, bciR and bciT arranged in an operon was
amplified using PCR, analyzed and submitted into the GenBank database (accession code, KR049081).
A two open reading frames (ORFs), namely BciR and BciT were found to encode a putative MerRtranscriptional
regulator (BciR; 153 aa) and a putative MFS transporter (BciT; 392 aa), respectively.
Analysis of the conserved domains and modeled tertiary structures revealed that, BciR possesses an Nterminal
H-T-H motive (HTH type) region with possible transcriptional related activity and a conserved
metal binding site at the C-terminal end. BciT was likely an MFS protein with nine transmembrane
helices. This is the first report about detection of a bciR/bciT operon that putatively encode a sensorresponse
transport system in Bacillus circulans ATCC 12588.
superfamily (MFS) transporters usually form an important sensor-response transport system in many
microorganisms. This system has been shown to be involved in the regulation and transport (efflux) of a
wide and diverse array of secondary metabolites including antimicrobial agents, dyes, chemicals,
metals and evem harmful oxygen radicals. Inhibition or inactivation of this transport system is
considered a promising approach for controlling microbial resistance, and thus may become a
promising target of therapeutics particularly for the clinically relevant pathogens. However, the genetic
and proteomics of this system have not been fully studied. In this work, a DNA segment (1.926 kb) from
Bacillus circulans ATCC 21588 harboring the two genes, bciR and bciT arranged in an operon was
amplified using PCR, analyzed and submitted into the GenBank database (accession code, KR049081).
A two open reading frames (ORFs), namely BciR and BciT were found to encode a putative MerRtranscriptional
regulator (BciR; 153 aa) and a putative MFS transporter (BciT; 392 aa), respectively.
Analysis of the conserved domains and modeled tertiary structures revealed that, BciR possesses an Nterminal
H-T-H motive (HTH type) region with possible transcriptional related activity and a conserved
metal binding site at the C-terminal end. BciT was likely an MFS protein with nine transmembrane
helices. This is the first report about detection of a bciR/bciT operon that putatively encode a sensorresponse
transport system in Bacillus circulans ATCC 12588.
Other data
Title | Isolation and sequence analysis of a putative MerR-type-transcriptional regulator and a multidrug efflux protein of Bacillus circulans ATCC 21588: as potential targets of therapeutics | Authors | Aboshanab K. ; Elshafey Mostafa | Keywords | MerR-type transcription regulator, multidrug efflux protein, major facilitator superfamily MFS, Bacillus circulans ATCC 21588 | Issue Date | 2015 | Journal | International Journal of Genetics and Molecular biology. 7(3): 15-24. | DOI | 10.5897/IJGMB2015.0112 |
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