Evaluation of Antidiabetic and Insulintropic Potential of Nigella Sativa Seeds Water Extract and /or Alpa Lipoic Acid in Type 2 Diabetic Rats
Mohamed, Enas Ali Kamel; Maha M. Mohamed;
Abstract
The objective of this study was to evaluate the possible antidiabetic and insulin tropic effect of nigella sativa seeds water extract and/or lipoic acid. For this purpose, Forty male Wistar strain albino rats were divided into five groups (8 rat /group), group 1; control group, group2; normal rats fed on basal diet and received 21% fructose in drinking water for four weeks then injected intraperitoneally by a single streptozotocin (STZ) dose (40 mg/kg body weight)to induce type2 diabetes and served as diabetic control group, group3; diabetic rats orally administrated water extract of nigella sativa (1ml/kg daily for 6 weeks), group4; diabetic rats orally administrated lipoic acid (100 mg/kg body weight daily for 6 weeks), group5;diabetic
rats orally administrated aqueous extract of nigella sativa and lipoic acid daily simultaneously at the previous tested doses for 6 weeks. Untreated diabetic group showed a significant increase in serum glucose, total cholesterol, triacylglycerols, transforming growth factor beta (TGF-β)and leptin. Also, hepatic total lipids, totalcholesterol, alcohol dehydrogenase (AD) activity were significantly elevated in untreated diabetic group as compared to normal control. Whereas, serum insulin, C-peptide, insulin like growth factor -1(IGF-1), hepatic glycogen and NADPH were significantly reduced in untreated diabetic group as compared to normal control. Moreover, the activities of hepatic enzymes cytochrome p450 reductase, glucose-6-phosphate dehydrogenase (G-6-P-D), Glyoxalase I and II were significantly reduced
in untreated diabetic rat as compared to normal control group (p<0.05).Oral administration of nigella sativa seeds water extract and /or lipoic acid restored all these altered biochemical parameters to near normal levels.
rats orally administrated aqueous extract of nigella sativa and lipoic acid daily simultaneously at the previous tested doses for 6 weeks. Untreated diabetic group showed a significant increase in serum glucose, total cholesterol, triacylglycerols, transforming growth factor beta (TGF-β)and leptin. Also, hepatic total lipids, totalcholesterol, alcohol dehydrogenase (AD) activity were significantly elevated in untreated diabetic group as compared to normal control. Whereas, serum insulin, C-peptide, insulin like growth factor -1(IGF-1), hepatic glycogen and NADPH were significantly reduced in untreated diabetic group as compared to normal control. Moreover, the activities of hepatic enzymes cytochrome p450 reductase, glucose-6-phosphate dehydrogenase (G-6-P-D), Glyoxalase I and II were significantly reduced
in untreated diabetic rat as compared to normal control group (p<0.05).Oral administration of nigella sativa seeds water extract and /or lipoic acid restored all these altered biochemical parameters to near normal levels.
Other data
Title | Evaluation of Antidiabetic and Insulintropic Potential of Nigella Sativa Seeds Water Extract and /or Alpa Lipoic Acid in Type 2 Diabetic Rats | Authors | Mohamed, Enas Ali Kamel ; Maha M. Mohamed | Keywords | Antidiabetic ;Insulintropic ;Nigella Sativa ;Alpa Lipoic Acid ; Type 2 Diabetic Rats | Issue Date | Nov-2015 | Publisher | Indian Journal of Medical Research and Pharmaceutical Sciences, 2(11):26-35, 2015 | Source | Indian Journal of Medical Research and Pharmaceutical Sciences, 2(11):26-35, 2015 | Journal | Indian Journal of Medical Research and Pharmaceutical Sciences, 2(11):26-35, 2015 |
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