Characterization of Crude Oil Degrading Bacteria Isolated from Contaminated Soils Surrounding Gas Stations
Abou-Shanab, Reda A I; Eraky, Mohamed; Haddad, Ahmed M; Abdel-Ghaffar, Abdel-Rahman; Salem, Ahmed M;
Abstract
A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Of the 20 isolates, RAM03, RAM06, RAM13, and RAM17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. These bacterial cultures had 16S rRNA gene sequences that were most similar to Ochrobactrum cytisi (RAM03), Ochrobactrum anthropi (RAM06 and RAM17), and Sinorhizobium meliloti (RAM13) with 96 %, 100 % and 99 %, and 99 % similarity. The tested strains revealed a promising potential for bioremediation of petroleum oil contamination as they could degrade >93 % and 54 % of total petroleum hydrocarbons (TPHs) in a liquid medium and soil amended with 4 % crude oil, respectively, after 30 day incubation. These bacteria could effectively remove both aliphatic and aromatic petroleum hydrocarbons. In conclusion, these strains could be considered as good prospects for their application in bioremediation of hydrocarbon contaminated environment.
Other data
Title | Characterization of Crude Oil Degrading Bacteria Isolated from Contaminated Soils Surrounding Gas Stations | Authors | Abou-Shanab, Reda A I ; Eraky, Mohamed ; Haddad, Ahmed M ; Abdel-Ghaffar, Abdel-Rahman ; Salem, Ahmed M | Issue Date | Nov-2016 | Publisher | SPRINGER | Journal | Bulletin of environmental contamination and toxicology | DOI | 684-688 1432-0800 5 688 https://api.elsevier.com/content/abstract/scopus_id/84988575945 97 10.1007/s00128-016-1924-2 |
PubMed ID | 27655077 | Scopus ID | 2-s2.0-84988575945 | Web of science ID | WOS:000387268000016 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.