MicroRNA-122 restoration modulates alpha-fetoprotein expression in hepatocellular carcinoma treated with doxorubicin
El-Toukhy S.; Fathy, Shadia; Yahya S.; El-Khayat Z.; Hegazy M.; Nabih H.;
Abstract
Higher levels of morbidity and mortality are due to malignant tumors and Hepatocellular carcinoma (HCC) is considered of a great percentage. The liver-specific microRNA-122 (mir-122) is frequently down-regulated in HCC and is a promising biomarker for the early HCC diagnosis. Restoration of mir-122 renders HCC cells to be sensitive to doxorubicin. The present study aims to investigate the effect of treatment of HCC cells with doxorubicin and/or mir-122 mimics on AFP expression levels. Using HepG2 cells with different treatments, AFP mRNAs and proteins were isolated and detected by real-time PCR and ELISA, respectively. Our data showed that there was a dynamic change in mir-122 expression as compared to inhibitor negative control. Also, there were no significant differences in AFP expression levels in cells treated with chronic doxorubicin dose then transfected with mir-122 mimics. However, our findings demonstrated statistical significant increase in both molecular and protein expression levels of AFP in cells treated with acute high doxorubicin dose and mir-122 mimics. This could be associated with increasing doxorubicin sensitivity by mir-122 restoration in HepG2 cells. This distinct finding could be attributed to a negative feedback increase in AFP levels to counteract the apoptosis/reactive oxygen species triggered by miR- 122 restoration in doxorubicin-treated HepG2 cells.
Other data
Title | MicroRNA-122 restoration modulates alpha-fetoprotein expression in hepatocellular carcinoma treated with doxorubicin | Authors | El-Toukhy S. ; Fathy, Shadia ; Yahya S. ; El-Khayat Z. ; Hegazy M. ; Nabih H. | Issue Date | 1-Jan-2016 | Journal | Der Pharmacia Lettre | DOI | 29 8 35 https://api.elsevier.com/content/abstract/scopus_id/84973468790 8 |
Scopus ID | 2-s2.0-84973468790 |
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