Tumor-facing hepatocytes significantly contribute to mild hyperthermia-induced targeting of rat liver metastasis by PLGA-NPs
Castellani, Chiara; Fedrigo, Marny; Tavano, Regina; Cappellini, Rekha; Fedeli, Chiara; Mognato, Maddalena; Abdel-Mottaleb, Mona; Lamprecht, Alf; Tudorancea, Ionut; Porumb, Vlad; Iliescu, Radu; Angelini, Annalisa; Papini, Emanuele; Dimofte, Gabriel;
Abstract
The effect of mild hyperthermia (MHT) on nanoparticle (NP) accumulation in rat model liver metastasis and the contribution of neoplastic and non-neoplastic cells were characterized. CdSe/ZnS QD-doped poly(lactic-co-glycolic acid) (PLGA) NPs (155 ± 10 nm) were delivered via the ileocolic vein to metastatic livers 15 min after localized MW irradiation (1 min, 41 °C) or in normothermia (37 °C, NT). Quantitative analysis of tissue sections by confocal fluorescence microscopy 1 h after NP injection showed no NP tumor accumulation in NT. On the contrary, MHT increased NP association with tumor, compared to normal tissue. Counterstaining of specific markers showed that the MHT effect is due to an increased NP endocytosis not only by tumor cells, but also by hepatocytes at the growing tumor edge and, to a minor extent, by tumor-associated macrophages. High-NP capturing hepatocytes, close to the tumor, may be a relevant phenomenon in MHT-induced increased targeting of NPs to liver metastasis, influencing their therapeutic efficacy.
Other data
Title | Tumor-facing hepatocytes significantly contribute to mild hyperthermia-induced targeting of rat liver metastasis by PLGA-NPs | Authors | Castellani, Chiara; Fedrigo, Marny; Tavano, Regina; Cappellini, Rekha; Fedeli, Chiara; Mognato, Maddalena; Abdel-Mottaleb, Mona ; Lamprecht, Alf; Tudorancea, Ionut; Porumb, Vlad; Iliescu, Radu; Angelini, Annalisa; Papini, Emanuele; Dimofte, Gabriel | Keywords | Hepatocytes;Liver cancer metastasis;Mild hyperthermia;PLGA-NPs;Tumor associated macrophages | Issue Date | 20-Jul-2019 | Publisher | ELSEVIER SCIENCE BV | Journal | International Journal of Pharmaceutics | Volume | 566 | Start page | 541 | End page | 548 | ISSN | 03785173 | DOI | 10.1016/j.ijpharm.2019.06.004 | PubMed ID | 31173801 | Scopus ID | 2-s2.0-85067256380 | Web of science ID | WOS:000472733600070 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.