Association of expression levels of pluripotency/stem cell markers with the differentiation outcome of Wharton's jelly mesenchymal stem cells into insulin producing cells
Kassem, Dina; El-Kholy, Abd El Latif G.; El-Mesallamy, Hala O.; Kamal, Mohamed;
Abstract
Recently, there has been much attention towards generation of insulin producing cells (IPCs) from stem cells, especially from Wharton's jelly mesenchymal stem cells (WJ-MSCs). However, generation of mature IPCs remains a challenge. Assessment of generation of IPCs was usually done by examining β-cell markers, however, assessment of pluripotency/stem cell markers drew less attention. Therefore, the purpose of this study was to investigate the levels of pluripotency/stem cell markers during differentiation of WJ-MSCs into IPCs and the association of these levels with differentiation outcomes. WJ-MSCs were isolated, characterized then induced to differentiate into IPCs using three different protocols namely A, B and C. Differentiated IPCs were assessed by the expression of pluripotency/stem cell markers, together with β-cell markers using qRT-PCR, and functionally by measuring glucose stimulated insulin secretion. Differentiated cells from protocol A showed lowest expression of pluripotency/stem cell markers and relatively best GSIS. However, protocol B showed concomitant expression of pluripotency/stem cell and β-cell markers with relatively less insulin secretion as compared to protocol A. Protocol C failed to generate glucose-responsive IPCs. In conclusion, sustained expression of pluripotency/stem cell markers could be associated with the incomplete differentiation of WJ-MSCs into IPCs. A novel finding for which further investigations are warranted.
Other data
Title | Association of expression levels of pluripotency/stem cell markers with the differentiation outcome of Wharton's jelly mesenchymal stem cells into insulin producing cells | Authors | Kassem, Dina ; El-Kholy, Abd El Latif G.; El-Mesallamy, Hala O.; Kamal, Mohamed | Keywords | Diabetes mellitus;Insulin producing cells;Mesenchymal stem cells;Pluripotency/stem cell markers;Wharton's jelly | Issue Date | 1-Aug-2016 | Publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | Journal | Biochimie | Volume | 127 | Start page | 187 | End page | 195 | ISSN | 03009084 | DOI | 10.1016/j.biochi.2016.05.019 | PubMed ID | 27265786 | Scopus ID | 2-s2.0-84974578113 | Web of science ID | WOS:000380082500021 |
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