Modern analytical techniques for early diagnosis of some chronic diseases

Abstract

A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of folic acid (FA) in pharmaceutical tablets, serum and urine samples using optical sensor Tb3+-3-ac. Based on its quenching effect on the luminescence intensity of Tb3+-3-ac complex as photo probe in acetonitrile at pH 8.2 and λex = 340 nm. The quenching of luminescence intensity Tb3+-3-ac complex especially the electrical band at λem = 545 nm is used for the assessment of FA in different body fluids. The dynamic range found for the determination of FA concentration is (1.28×10-6 – 2.49 × 10-9) mol L−1, ( r = 0.999 ). The detection limit (S:N=3) ( LOD) and quantitation limit of detection (LOQ) are (4.0 ×10−9and 1.21×10-8) mol L-1 respectively . This method was simple, practical and relatively free from interference effect. It was successfully applied to assess FA in pharmaceutical tablets, serum and urine samples.