Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli
Abou-Taleb, Khadiga A.A., Mashhoor, W.A., Nasr, Sohair A.,Sharaf, M.S. and Abdel-Azeem, Hoda H.M.; Abou-Taleb, Khadiga;
Abstract
Effect of some nutritional and environmental factors on growth and cellulase production
by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated
that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and
nitrogen sources respectively. Initial pH 7 and 3% inoculum size found to be optimal for growth and
cellulase production. Incubation temperature at 30 and 45ºC achieved the highest activity of cellulase
for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking
rate was 150 and 200 rpm.
by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated
that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and
nitrogen sources respectively. Initial pH 7 and 3% inoculum size found to be optimal for growth and
cellulase production. Incubation temperature at 30 and 45ºC achieved the highest activity of cellulase
for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking
rate was 150 and 200 rpm.
Other data
| Title | Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli | Authors | Abou-Taleb, Khadiga A.A., Mashhoor, W.A., Nasr, Sohair A.,Sharaf, M.S. and Abdel-Azeem, Hoda H.M. ; Abou-Taleb, Khadiga | Keywords | Nut ritional & environmental factors, Cellulas e production, B. alcalophilus S39, B. amyloliquefaciens C23. | Issue Date | 2009 | Publisher | INSInet | Source | Abou-Taleb, Kh. A.A., Mashhoor, W.A., Nasr, S. A., Sharaf, M.S. and Abdel-Azeem, H. H.M. (2009). Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli. Australian Journal of Basic and Applied Sciences, 3(3): 2429-2436 | Journal | Aus tralian Journal of Bas ic and Applied Sciences | Description | Cellulase production by bacteria |
Attached Files
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| Cellulase (1).pdf | 325.37 kB | Adobe PDF | Request a copy |
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