CELLULASES PRODUCTION BY TWO STRAINS OF CELLULOLYTIC BACILLI USING VARIOUS AGRICULTURE WASTES
Abou-Taleb, Khadiga; W. A. Mashhoor; Sohair, A. Nasr; M. S. Sharaf;
Abstract
Some agricultural by-products and wastes such as sugar cane
bagasse, corncobs, rice straw, saw dust and wheat bran were
investigated as carbon cellulosic sources for production of cellulase, it
was found that sugar cane bagasse proved to be the best agricultural
waste during 4 days of incubation period for Bacillus alcalophilus S39
and B. amyloliquefaciens C23 in submerged culture after being added to
the basal medium. However in solid-state cultivations it was found that
sugar cane bagasse was the best waste, achieved after 7 days of
incubation period by Bacillus alcalophilus S39 and B. amyloliquefaciens
C23. The addition of by-product such as corn steep liquor (CSL) was
used as nitrogen source gave the lowest cellulases yield in basal
medium and the medium contain cellulosic waste as carbon source. A
low price productive medium composed of (sugar cane bagasse as
carbon source, yeast extract as nitrogen source & a mixture of
elements) was suggested for cellulases production by Bacillus
alcalophilus S39 grown in shake flasks as a batch culture for 4 under
controlled environmental conditions. The highest cellulases production
was achieved after 66 h by Bacillus alcalophilus S39 under controlled
environmental conditions using bioreactor as a batch culture. This
showed the highest cellulases production 5 % as compared with flaks
as a batch culture.
bagasse, corncobs, rice straw, saw dust and wheat bran were
investigated as carbon cellulosic sources for production of cellulase, it
was found that sugar cane bagasse proved to be the best agricultural
waste during 4 days of incubation period for Bacillus alcalophilus S39
and B. amyloliquefaciens C23 in submerged culture after being added to
the basal medium. However in solid-state cultivations it was found that
sugar cane bagasse was the best waste, achieved after 7 days of
incubation period by Bacillus alcalophilus S39 and B. amyloliquefaciens
C23. The addition of by-product such as corn steep liquor (CSL) was
used as nitrogen source gave the lowest cellulases yield in basal
medium and the medium contain cellulosic waste as carbon source. A
low price productive medium composed of (sugar cane bagasse as
carbon source, yeast extract as nitrogen source & a mixture of
elements) was suggested for cellulases production by Bacillus
alcalophilus S39 grown in shake flasks as a batch culture for 4 under
controlled environmental conditions. The highest cellulases production
was achieved after 66 h by Bacillus alcalophilus S39 under controlled
environmental conditions using bioreactor as a batch culture. This
showed the highest cellulases production 5 % as compared with flaks
as a batch culture.
Other data
Title | CELLULASES PRODUCTION BY TWO STRAINS OF CELLULOLYTIC BACILLI USING VARIOUS AGRICULTURE WASTES | Authors | Abou-Taleb, Khadiga ; W. A. Mashhoor; Sohair, A. Nasr; M. S. Sharaf | Keywords | Cellulases;agro-industrial wastes;Bacillus sp;Bioreactor | Issue Date | 2012 | Journal | J. Biol. Chem. Environ. Sci. | Volume | 7 | Issue | 4 | Start page | 93 | End page | 107 |
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