Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats
Afaf Abbass Sayed Saleh;
Abstract
To clarify the protective effects of Taurine (TA) and Coenzyme Q10 (CoQ10) for mitigation of
acrylamide- induced oxidative damage. .
Method: Acrylamide (AA), TA and CoQ10 were administered orally to rats for 2 and 4 weeks. Sixty
albino rats of either sex weighing 200 ± 5 were randomly divided into five groups; control group, AA
group, AA+ TA group, AA+ CoQ10 group and AA+ TA+ CoQ10 Group ( 15, 500 and 200 mg/kg/day
dose, respectively). Hepatoprotection was assessed. The level of hepatic marker enzymes including
serum lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT)
were evaluated. The proinflammatory cytokines including serum levels of tumor necrosis factor-α (TNF-α), interleukin- 1β (IL- 1β) and interleukin-6(IL-6) were assessed. The levels of reduced glutathione
(GSH), malondialdehyde (MDA) and myeloperoxidase (MPO) in liver homogenate were also performed.
Results: TA or CoQ10 significantly decreased (p < 0.05) the elevation of hepatic markers (LDH, AST
and ALT) induced by AA in rats. Reduction of serum proinflammatory cytokines (TNF-α, IL- 1β and IL-6)
were also observed compared to AA group, and it was a time-effect relationship. Treatment with TA or
CoQ10 also significantly reduced the oxidative stress induced by AA as shown by an increase in GSH
level, and reduction of MDA level and MPO activities compared to rats treated with AA alone (p < 0.05).
The hepatoprotective effect of both TA and CoQ10 combination was more efficient than the effect of
either of them alone.
Conclusion: The combination of TA and Co Q10 possesses a good potential to inhibit oxidative stress
from liver and also exhibits anti-inflammatory activity. Thus, they have the potential to be used to
mitigate AA- induced liver injury
acrylamide- induced oxidative damage. .
Method: Acrylamide (AA), TA and CoQ10 were administered orally to rats for 2 and 4 weeks. Sixty
albino rats of either sex weighing 200 ± 5 were randomly divided into five groups; control group, AA
group, AA+ TA group, AA+ CoQ10 group and AA+ TA+ CoQ10 Group ( 15, 500 and 200 mg/kg/day
dose, respectively). Hepatoprotection was assessed. The level of hepatic marker enzymes including
serum lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT)
were evaluated. The proinflammatory cytokines including serum levels of tumor necrosis factor-α (TNF-α), interleukin- 1β (IL- 1β) and interleukin-6(IL-6) were assessed. The levels of reduced glutathione
(GSH), malondialdehyde (MDA) and myeloperoxidase (MPO) in liver homogenate were also performed.
Results: TA or CoQ10 significantly decreased (p < 0.05) the elevation of hepatic markers (LDH, AST
and ALT) induced by AA in rats. Reduction of serum proinflammatory cytokines (TNF-α, IL- 1β and IL-6)
were also observed compared to AA group, and it was a time-effect relationship. Treatment with TA or
CoQ10 also significantly reduced the oxidative stress induced by AA as shown by an increase in GSH
level, and reduction of MDA level and MPO activities compared to rats treated with AA alone (p < 0.05).
The hepatoprotective effect of both TA and CoQ10 combination was more efficient than the effect of
either of them alone.
Conclusion: The combination of TA and Co Q10 possesses a good potential to inhibit oxidative stress
from liver and also exhibits anti-inflammatory activity. Thus, they have the potential to be used to
mitigate AA- induced liver injury
Other data
Title | Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats | Authors | Afaf Abbass Sayed Saleh | Keywords | Taurine, Coenzyme Q10, Acrylamide, Oxidative stress, Biochemical profile, Proinflammatory cytokines | Issue Date | 16-Aug-2017 | Publisher | Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. | Journal | Tropical Journal of Pharmaceutical Research | DOI | 10.4314/tjpr.v16i8.14 Original Research Article |
Attached Files
File | Description | Size | Format | Existing users please Login |
---|---|---|---|---|
6.pdf | 393.72 kB | Adobe PDF | Request a copy |
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.