A Short Peptide Designed from Late Embryogenesis Abundant Protein Enhances Acid Tolerance in Escherichia coli
Metwally, Khaled; Ikeno, Shinya;
Abstract
Unsuitable pH is a major limiting factor for all organisms, and a low pH can lead to
organism death. Late embryogenesis abundant (LEA) peptides confer tolerance to abiotic
stresses including salinity, drought, high and low temperature, and ultraviolet radiation
same as the LEA proteins from which they originate. In this study, LEA peptides derived
from group 3 LEA proteins of Polypedilum vanderplanki were used to enhance low pH
tolerance. Recombinant Escherichia coli BL21 (DE3) cells expressing the five designed
LEA peptides were grown at pH 4, 3, and 2. The transformants showed higher growth
capacity at low pH as compared to control cells. These results indicate that LEA peptide
could prevent E. coli cell death under low pH conditions.
organism death. Late embryogenesis abundant (LEA) peptides confer tolerance to abiotic
stresses including salinity, drought, high and low temperature, and ultraviolet radiation
same as the LEA proteins from which they originate. In this study, LEA peptides derived
from group 3 LEA proteins of Polypedilum vanderplanki were used to enhance low pH
tolerance. Recombinant Escherichia coli BL21 (DE3) cells expressing the five designed
LEA peptides were grown at pH 4, 3, and 2. The transformants showed higher growth
capacity at low pH as compared to control cells. These results indicate that LEA peptide
could prevent E. coli cell death under low pH conditions.
Other data
Title | A Short Peptide Designed from Late Embryogenesis Abundant Protein Enhances Acid Tolerance in Escherichia coli | Authors | Metwally, Khaled ; Ikeno, Shinya | Keywords | Escherichia coli . LEA peptide . Abiotic stress. Low pH . Late embryogenesis abundant protein | Issue Date | 25-Feb-2020 | Publisher | Applied Biochemistry and Biotechnology | Journal | Applied biochemistry and biotechnology | Volume | 191 | Start page | 164 | End page | 176 | DOI | https://doi.org/10.1007/s12010-020-03262-5 |
Attached Files
File | Description | Size | Format | Existing users please Login |
---|---|---|---|---|
Metwally-Ikeno2020_Article_AShortPeptideDesignedFromLateE (1).pdf | 590.84 kB | Adobe PDF | Request a copy |
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.