Wound healing activity of opuntia ficus-indica fixed oil formulated in a self-nanoemulsifying formulation
Koshak, Abdulrahman E.; Algandaby, Mardi M.; Mujallid, Mohammad I.; Abdel-Naim, Ashraf B.; Alhakamy, Nabil A.; Fahmy, Usama A.; Alfarsi, Anas; Badr-Eldin, Shaimaa M.; Neamatallah, Thikryat; Nasrullah, Mohammed Z.; Abdallah, Hossam M.; Ahmed Esmat;
Abstract
Introduction: Delayed wound healing represents a common health hazard. Traditional herbal products have been often utilized to promote wound contraction. The current study aimed at assessing the wound healing activity of Opuntia ficus-indica seed oil (OFI) and its self-nanoemulsifying drug delivery system (OFI-SNEDDS) formula in a rat model of fullthickness skin excision. Methods: Based on droplet size, an optimized OFI-SNEDDS formula was prepared and used for subsequent evaluation. Wound healing activity of OFI and OFI-SNEDDS was studied in vivo. Results: The optimized OFI-SNEDDS formula droplet size was 50.02 nm. The formula exhibited superior healing activities as compared to regular OFI seed oil-treated rats at day 14 of wounding. This effect was further confirmed by histopathological examinations of H&E and Masson’s Trichrome-stained skin sections. Moreover, OFI-SNEDDS showed the highest antioxidant and anti-inflammatory activities as compared to OFI seed oil-treated animals. Both OFI and OFI-SNEDDS significantly enhanced hydroxyproline skin content and upregulated Col1A1 mRNA expression, accompanied by enhanced expression of transforming factor-beta (TGF-β). Further, OFI-SNEDDS improved angiogenesis as evidenced by increased expression of vascular endothelial growth factor (VEGF). Conclusion: OFI possesses wound healing properties that are enhanced by self-emulsification of the oil into nano-droplets. The observed activity can be attributed, at least partly, to its anti-inflammatory, pro-collagen and angiogenic properties.
Other data
Title | Wound healing activity of opuntia ficus-indica fixed oil formulated in a self-nanoemulsifying formulation | Authors | Koshak, Abdulrahman E.; Algandaby, Mardi M.; Mujallid, Mohammad I.; Abdel-Naim, Ashraf B.; Alhakamy, Nabil A.; Fahmy, Usama A.; Alfarsi, Anas; Badr-Eldin, Shaimaa M.; Neamatallah, Thikryat; Nasrullah, Mohammed Z.; Abdallah, Hossam M.; Ahmed Esmat | Keywords | Opuntia ficus-indica;Wound healing;VEGF;TGF-β;SNEDDS;Rats | Issue Date | 1-Jan-2021 | Publisher | DOVE MEDICAL PRESS LTD | Journal | International Journal of Nanomedicine | ISSN | 11769114 | DOI | 10.2147/IJN.S299696 | PubMed ID | 34135583 | Scopus ID | 2-s2.0-85108346850 | Web of science ID | WOS:000660805800001 |
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