High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4 -induced hepatic damage
Sobeh, Mansour; Youssef, Fadia S.; Ahmed Esmat; Petruk, Ganna; El-Khatib, Ahmed H.; Monti, Daria Maria; Ashour, Mohamed L.; Wink, Michael;
Abstract
Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug.
Other data
Title | High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl<inf>4</inf>-induced hepatic damage | Authors | Sobeh, Mansour; Youssef, Fadia S.; Ahmed Esmat ; Petruk, Ganna; El-Khatib, Ahmed H.; Monti, Daria Maria; Ashour, Mohamed L. ; Wink, Michael | Keywords | Antioxidant;Carbon tetrachloride toxicity;HaCaT cells;Hepatoprotective activity;HR-UPLC-MS/MS;Syzygium samarangense | Issue Date | 1-Mar-2018 | Publisher | PERGAMON-ELSEVIER SCIENCE LTD | Journal | Food and Chemical Toxicology | Volume | 113 | Start page | 145 | End page | 153 | ISSN | 02786915 | DOI | 10.1016/j.fct.2018.01.031 | PubMed ID | 29374594 | Scopus ID | 2-s2.0-85041563585 | Web of science ID | WOS:000428498100016 |
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