Eucalyptus Sideroxylon Bark Anti-inflammatory Potential, Its UPLC-PDA-ESI-qTOF-MS Profiling, and Isolation of a New Phloroglucinol
Ashour, Rehab M S; Okba, Mona M; Menze, Esther; El Gedaily, Rania A;
Abstract
Eucalyptus barks contain complex biomass of constituents with considerable chemical and structural diversity. Reports about Eucalyptus sideroxylon Cunn. ex Woolls bark composition and biological activities are limited. Non-targeted metabolomic analysis via ultra-performance liquid chromatography-quadrupole-time-of-flight-photodiode array-mass spectrometry (UPLC-qTOF-PDA-MS) enabled first-time detection of 41 secondary metabolites of which 31 were identified including; 6 flavonoids, 4 ellagic acid derivatives, 8 triterpenes, 10 fatty acids and 3 miscellaneous. The isolation and structure elucidation of methyl morolate, β-sitosterol, syringaldeyhde and 7'-deoxyguajavadial A were reported. The bark methylene chloride: methanol (8:2) extract demonstrated significant (P < 0.01) in vitro anti-inflammatory activity through membrane stabilization, protein denaturation inhibition, anti-lipoxygenase, and proteinase inhibition assays. The strongest anti-inflammatory activity was via membrane stabilization (34.4%) as compared to diclofenac sodium (26%) at the same concentration (125 μg/mL). Our study represents the sole complete map for E. sideroxylon bark components and represents it as new anti-inflammatory drug.
Other data
Title | Eucalyptus Sideroxylon Bark Anti-inflammatory Potential, Its UPLC-PDA-ESI-qTOF-MS Profiling, and Isolation of a New Phloroglucinol | Authors | Ashour, Rehab M S; Okba, Mona M; Menze, Esther ; El Gedaily, Rania A | Keywords | deoxyguajavadial A;methyl morolate;membrane stabilization;UPLC-MS | Issue Date | 1-Jul-2019 | Publisher | OXFORD UNIV PRESS INC | Journal | Journal of chromatographic science | Volume | 57 | Issue | 6 | Start page | 565 | End page | 574 | ISSN | 0021-9665 | DOI | 10.1093/chromsci/bmz029 | PubMed ID | 31209500 | Scopus ID | 2-s2.0-85068426527 | Web of science ID | WOS:000482496800010 |
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