Methicillin-resistant Staphylococcus aureus (MRSA) isolated from chicken meat and giblets often produces staphylococcal enterotoxin B (SEB) in non-refrigerated raw chicken livers
Abolghait, Said; Fathi, Amal Gamal; Youssef, Fatma Mohamed; Algammal, Abdelazeem Mohamed;
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections and staphylococcal food poisoning (SFP). This study was conducted to investigate the prevalence and enterotoxigenicity of MRSA in broiler chicken meat and giblets. A total of 5.5% (8/144) of the examined samples were contaminated with mecA positive/mecC negative MRSA, with staphylococcal counts of approximately 102 colony forming units (CFU)/g in breast, leg and gizzard samples and approximately 3.3 × 103 CFU/g in frozen liver samples. Most MRSA isolates (75%, 6/8) harboured the staphylococcal enterotoxin B (seb) gene. Reverse transcription-PCR (RT-PCR) showed that MRSA isolates initiated SEB production in experimentally contaminated chicken livers within 24 h of storage at temperatures over 8 °C. SEB was maximally produced at 24 °C when the MRSA counts reached 7.3 × 103 ± 1.2 × 103 CFU/g sample homogenate. The current study concludes that the main broiler chicken MRSA isolates in Egypt harbour the seb gene. To mitigate possible SEB production, especially in broiler chicken livers, a maximum “out of refrigeration” time limit should be implemented for cold chain poultry products.
Other data
Title | Methicillin-resistant Staphylococcus aureus (MRSA) isolated from chicken meat and giblets often produces staphylococcal enterotoxin B (SEB) in non-refrigerated raw chicken livers | Authors | Abolghait, Said ; Fathi, Amal Gamal; Youssef, Fatma Mohamed; Algammal, Abdelazeem Mohamed | Keywords | Chicken meat and giblets;enterotoxin expression;Liver;MRSA;Refrigeration;SEB | Issue Date | 2-Sep-2020 | Journal | International Journal of Food Microbiology | Volume | 328 | ISSN | 01681605 | DOI | 10.1016/j.ijfoodmicro.2020.108669 | PubMed ID | 32497922 | Scopus ID | 2-s2.0-85085610036 |
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