Biochemical studies of captopril against 5-Flurouracil induced heart Toxicity in rats

Abstract

Captopril (CAP)is an angiotensin converting enzyme inhibitor and the aim of the present study examined whether, captopril exert beneficial effect on 5-fluorouracil (5-FLU) induced heart toxicity. In the present study twenty four male Sprague-dawley rats were assigned to four equal groups: Group I: rats received normal saline solution (control group); Group II:rats received 5-FLU 20 mg /kg/day, intraperitoneally for 5 days (5-FLU group); Group III: rats received captopril 20 mg /kg/day, orally for 14 days (captopril group); Group IV:rats received 5-FU 20 mg /kg/day, intraperitoneally for 5 days + captopril 20 mg /kg/day, orally for 14 days (5-FLU + captopril group). At the end of experiment animals of all groups were sacrificed, blood and tissue samples from the aorta artery were taken and used for the determination of the investigated biochemical parameters.Injection of 5-FLU causeda significant increase in serum lactate dehydrogenase(LDH) activity and increase in relative heart weight to body weight, serum total cholesterol(TC),triglycerides(TG),low density lipoprotein(LDL), high density lipoprotein(HDL) and malondialdehyde (MDA). In additional, reduced in serum glutathione-s-transferase (GST) was evoked. mineral study showed a significant increase in serum calcium ion (Ca ++) and in sodium ion Na+ but decrease concentration of K+ was noticed compared to control groups.The histopathological study revealed that administration of 5-FLU induced marked alteration in Aortic tissueartery; these included focal inflammatory cells, vacuolar in tunica media, highmagnification and hyalinization.On other hand, administration of CAP and/or 5-FLU, showed improvement in biochemical and histological alterations by decreasing lipid profile, lactate dehydrogenase,lipid oxidation, increasing in GST content and improved the mineral content as well as improving aortic endothelium intima.The present study showed that concomitant captopril use might prevent the side effects of 5- Fluorouracil induced heart toxicity and may be correlated to its antioxidative action of captopril.