The Potential Role of Bone Marrow-Mesenchymal Stem Cells on Sodium Nitrite - Hypoxia Model in Liver of Male Rats.

Abstract

A limited oxygen supply leads to hepato-cellular injure and cell death. Sodium nitrite induced hypoxia and hepatic damage. The current work aims to examine the potential role of stem cells on biochemical markers, histopathological and ultrastructure alterations of hypoxia caused by sodium nitrite (NaNO2) toxicity in the liver of male rats. Adult male rats were divided into 6 groups. Group 1 (G1) was the control group. Group 2 (G2) received daily NaNO2 (35 mg/kg bwt/ day) via subcutaneous injection for 3 weeks. Group 3 (G3) received NaNO2 for 2 weeks and were then injected once with 2x106 mesenchymal stem cells (MSCs) intravenously and sacrificed 4 weeks later. Group 4 (G4) treated as group 3 followed by daily NaNO2 injection for 1 week; rats in G4 were sacrificed 4 weeks from MSCs treatment. Group 5 (G5) rats were treated with NaNO2 for 2 weeks and then left to recover for 4 weeks. Finally, Group 6 (G6) rats were treated with NaNO2 for 3 weeks and left to recover for 3 weeks, after which point they were sacrificed. The results showed that NaNO2 caused oxidative damage and histopathological alterations in the rat liver, as well as increased the levels of liver oxidative stress markers in comparison with the control group. Nevertheless, the administration of stem cells reduced the danger actions of sodium nitrite by enhancing biochemical marker concentration. The definite of improvement of histo-pathological lesions is indicted in response to MSCs therapy. Keywords: MSCs; Liver; NaNO2 toxicity; Oxidative stress; Liver histology.