Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics
Safa S. Hafez; Elham E. Moustafa; Gebrael, Sanaa; Abdallah, Soad A.;
Abstract
Klebsiella pneumoniae carbapenemase producing bacteria are
defined as a group of Gram-negative bacilli that are highly resistant
to drugs. The resistance of pathogens of the
Enterobacteriaceae family to β-lactam antibiotics such as carbapenems
is considered a major threat in the medical field. The
main aim of the current study was to explore and confirm the
occurrence of carbapenemase producing K. pneumoniae in the
Intensive Care Unit (ICU) in different hospital environment sample
sites of two Egyptian hospitals in Cairo. Isolates were collected
and the screening criteria of carbapenemase producing bacteria
was followed by the investigators in order to record the antimicrobial
resistance patterns of all isolates in addition to the molecular
identification using the 16S rDNA.
This study determined the sites responsible for the spreading
of carbapenem-resistant K. pneumoniae including suction tubes,
ventilator tubes, instrument tables and beds. All K. pneumoniae
isolates collected from the ICU of both hospitals were resistant to
oxacillin, meropenem and ceftazidime. Moreover, 16S rDNA gene
sequence was used to study bacterial phylogeny and taxonomy for
all K. pneumoniae isolates; the accession number of all isolates is
reported. We concluded that infection control department policies
in each hospital should be reinforced to avoid the escalation of K.
pneumoniae as nosocomial infections in hospitals. This study
should be repeated in other hospitals (especially the public hospitals)
to assess the level of the problem.
defined as a group of Gram-negative bacilli that are highly resistant
to drugs. The resistance of pathogens of the
Enterobacteriaceae family to β-lactam antibiotics such as carbapenems
is considered a major threat in the medical field. The
main aim of the current study was to explore and confirm the
occurrence of carbapenemase producing K. pneumoniae in the
Intensive Care Unit (ICU) in different hospital environment sample
sites of two Egyptian hospitals in Cairo. Isolates were collected
and the screening criteria of carbapenemase producing bacteria
was followed by the investigators in order to record the antimicrobial
resistance patterns of all isolates in addition to the molecular
identification using the 16S rDNA.
This study determined the sites responsible for the spreading
of carbapenem-resistant K. pneumoniae including suction tubes,
ventilator tubes, instrument tables and beds. All K. pneumoniae
isolates collected from the ICU of both hospitals were resistant to
oxacillin, meropenem and ceftazidime. Moreover, 16S rDNA gene
sequence was used to study bacterial phylogeny and taxonomy for
all K. pneumoniae isolates; the accession number of all isolates is
reported. We concluded that infection control department policies
in each hospital should be reinforced to avoid the escalation of K.
pneumoniae as nosocomial infections in hospitals. This study
should be repeated in other hospitals (especially the public hospitals)
to assess the level of the problem.
Other data
Title | Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics | Authors | Safa S. Hafez ; Elham E. Moustafa ; Gebrael, Sanaa ; Abdallah, Soad A. | Keywords | Intensive care unit pathogens; Inanimate sources; Antibiotic resistant Klebsiella pneumoniae; Carbapenemase. | Issue Date | 7-Jun-2018 | Publisher | scopus | Journal | Journal of Biological Research | DOI | 10.4081/jbr.2018.7437 |
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