Revisiting the Role of the Myofibroblast in Socket Surgery: An Immunohistochemical Study
Rashed, Hazem; Abdulhafez M.; Fouad Y.; Tawfik H.; Osman W.;
Abstract
© Copyright 2016 The American Society of Ophthalmic Plastic and Reconstructive Surgery, Inc. Purpose: To determine the impact of a single injection of various anti-inflammatory, antimitotic, and antiangiogenic agents on the cell count of myofibroblasts in an eviscerated socket. Methods: One eye from 15 skeletally mature New Zealand white rabbits was eviscerated, and the rabbits were divided into 5 groups. Each group of 3 rabbits received a 0.1 ml subconjunctival injection of a different agent. Group I received bevacizumab 25 mg/ml, group II received triamcinolone 40 mg/ml, group III received 5-fluorouracil 50 mg/ml, group IV received mitomycin-C 0.4 mg/ml, while group V was the control group and received no injections. The animals were euthanized 19 days after evisceration and conjunctival samples were submitted for histopathological examination. Monoclonal α-smooth muscle actin antibody was applied, and the mean of 5 readings of the number of myofibroblasts was recorded in each slide. Results: The mean count of myofibroblasts was highest for the control group and all groups achieved a statistically significant reduction in myofibroblast count compared with the control group. Sorting the means showed that Group IV (mitomycin-C) achieved the lowest mean value (p = 0.000006) followed by triamcinolone (p = 0.00048), while group I (bevacizumab) achieved the least reduction in myofibroblast count (p = 0.00148). Conclusion: Until newer antimyofibroblast medications and antibodies are commercially available, a single injection of mitomycin-C or triamcinolone during surgery achieves the highest mean reduction of myofibroblast count.
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Title | Revisiting the Role of the Myofibroblast in Socket Surgery: An Immunohistochemical Study | Authors | Rashed, Hazem ; Abdulhafez M. ; Fouad Y. ; Tawfik H. ; Osman W. | Issue Date | 1-Aug-2016 | Journal | Ophthalmic Plastic and Reconstructive Surgery | DOI | 4 292 http://api.elsevier.com/content/abstract/scopus_id/84978699107 32 1537-2677 10.1097/IOP.0000000000000510 |
PubMed ID | 32 | Scopus ID | 2-s2.0-84978699107 |
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